Plant cryopreservation

Plant cryopreservation is a genetic resource conservation strategy that allows plant material, such as seeds, pollen, shoot tips or dormant buds to be stored indefinitely in liquid nitrogen.[1] After thawing, these genetic resources can be regenerated into plants and used on the field. While this cryopreservation conservation strategy can be used on all plants, it is often only used under certain circumstances: 1) crops with recalcitrant seeds e.g. avocado,[2] coconut[3] 2) seedless crops such as cultivated banana and plantains[4] or 3) crops that are clonally propagated such as cassava, potato, garlic and sweet potato.[5][6]

  1. ^ Reed BM (2017-08-01). "Plant cryopreservation: a continuing requirement for food and ecosystem security". In Vitro Cellular & Developmental Biology - Plant. 53 (4): 285–288. doi:10.1007/s11627-017-9851-4. ISSN 1475-2689. S2CID 32177737.
  2. ^ O'Brien C, Hiti-Bandaralage JC, Folgado R, Lahmeyer S, Hayward A, Mitter N (2020). "Developing a cryopreservation protocol for avocado ( Persea americana Mill.) apical shoot tips using different antioxidants". Acta Horticulturae (1285): 15–22. doi:10.17660/ActaHortic.2020.1285.3. ISSN 0567-7572. S2CID 226751510.
  3. ^ Sopade PA, Samosir YM, Rival A, Adkins SW (December 2010). "Dehydration improves cryopreservation of coconut (Cocos nucifera L.)". Cryobiology. 61 (3): 289–96. doi:10.1016/j.cryobiol.2010.09.007. PMID 20959171.
  4. ^ Panis B, Swennen R (1995). "Cryopreservation of Germplasm of Banana and Plantain (Musa Species)". Cryopreservation of Plant Germplasm I. Biotechnology in Agriculture and Forestry. Vol. 32. Berlin, Heidelberg: Springer Berlin Heidelberg. pp. 381–397. doi:10.1007/978-3-662-03096-7_27. ISBN 978-3-642-08184-2.
  5. ^ Wilms H, Fanega Sleziak N, Van der Auweraer M, Brands M, Verleije M, Hardeman D, et al. (7 September 2020). "Development of a fast and user-friendly cryopreservation protocol for sweet potato genetic resources". Scientific Reports. 10 (1): 14674. Bibcode:2020NatSR..1014674W. doi:10.1038/s41598-020-70869-3. PMC 7477159. PMID 32895398.
  6. ^ Zimnoch-Guzowska E., Chmielarz P., Wawrzyniak M.K., Plitta-Michalak B.P., Michalak M., Pałucka M., Wasileńczyk U., Kosek P., Kulus D., Rucińska A., Mikuła A., 2022. Polish cryobanks: research and conservation for plant genetic resources. Acta Societatis Botanicorum Poloniae 91: 9121. https://doi.org/10.5586/asbp.9121