Polygalacturonase

Endo-polygalacturonase
Computer generated image of Polygalacturonase as found in Aspergillus aculeatus (1IA5) at pH 8.5[1]
Identifiers
EC no.3.2.1.15
CAS no.9032-75-1
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IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
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Endo-polygalacturonase (EC 3.2.1.15, pectin depolymerase, pectolase, pectin hydrolase, and poly-α-1,4-galacturonide glycanohydrolase; systematic name (1→4)-α-D-galacturonan glycanohydrolase (endo-cleaving)) is an enzyme that hydrolyzes the α-1,4 glycosidic bonds between galacturonic acid residues:

(1,4-α-D-galacturonosyl)n+m + H2O = (1,4-α-D-galacturonosyl)n + (1,4-α-D-galacturonosyl)m

Polygalacturonan, whose major component is galacturonic acid,[2] is a significant carbohydrate component of the pectin network that comprises plant cell walls.[3] Therefore, the activity of the endogenous plant PGs works to soften and sweeten fruit during the ripening process. Similarly, phytopathogens use PGs as a means to weaken the pectin network, so that digestive enzymes can be excreted into the plant host to acquire nutrients.

  1. ^ Cho SW, Lee S, Shin W (August 2001). "The X-ray structure of Aspergillus aculeatus polygalacturonase and a modeled structure of the polygalacturonase-octagalacturonate complex". Journal of Molecular Biology. 311 (4): 863–78. doi:10.1006/jmbi.2001.4919. PMID 11518536.
  2. ^ "Medical Definition of Polygalacturonan". Lexic.us.
  3. ^ Jones, T. M., Anderson, A. J., and Albersheim, P. (1972) Host–pathogen interactions IV, Studies on the polysaccharide-degrading enzymes secreted by Fusarium oxysporum f. sp. lycopersici, Physiol. Plant Pathol. 2, 153-166.