Polynucleobacter asymbioticus

Polynucleobacter asymbioticus
Scientific classification
Domain:
Bacteria
Phylum:
Pseudomonadota
Class:
Betaproteobacteria
Order:
Burkholderiales
Family:
Burkholderiaceae
Genus:
Polynucleobacter
Species:
P. asymbioticus
Binomial name
Polynucleobacter asymbioticus
Hahn et al. 2016
Type strain
QLW-P1DMWA-1, DSM 18221, CIP 109841[1]
Synonyms

Polynucleobacter necessarius subsp. asymbioticus, Polynucleobacter sp. QLW-P1DMWA-1, strain QLW-P1DMWA-1

Polynucleobacter asymbioticus is an aerobic, catalase- and oxidase-positive, chemo-organotrophic, nonmotile, free-living bacterium of the genus Polynucleobacter. The type strain was isolated from a small pond located in the Austrian Alps in the area of Salzburg[2] and described as a new subspecies of Polynucleobacter necessarius in 2009.[3] The classification of the type strain was hampered by the fact that its closest described relative represented obligate endosymbionts, i.e. P. necessarius, not available as a pure culture suitable for standard tests (DNA-DNA hybridization experiments) for delineation of prokaryotic species. Therefore, the strain was preliminarily placed in the subspecies P. necessarius subsp. asymbioticus. Later sequencing of the genome of the type strain[4] revealed that the strain represented a novel species within the genus Polynucleobacter. Therefore, its taxonomic rank was lifted from the subspecies to the species level.[5] Strains of P. asymbioticus dwell as planktonic organisms in acidic, humic-rich freshwater systems.[6] Comparative genome analyses revealed that P. asymbioticus represents an atypical member of the family Burkholderiaceae regarding its small genome size and its passive lifestyle.[7] A recent study used a collection of 37 P. asymbioticus strains isolated from various ponds located in a larger region of the Austrian Alps to gain insights in the evolution of Polynucleobacter bacteria.[8]

  1. ^ Straininfo of Polynucleobacter necessarius subsp. asymbioticus
  2. ^ Hahn, M.W., Pöckl, M. and Wu, Q.L. (2005) Low intraspecific diversity in a Polynucleobacter subcluster population numerically dominating bacterioplankton of a freshwater pond. Appl. Environ. Microbiol. 71: 4539–4547.
  3. ^ Hahn, M.W., Lang, E., Brandt, U., Wu, Q.L., and Scheuerl, T. (2009) Emended description of the genus Polynucleobacter and the species P. necessarius and proposal of two subspecies, P. necessarius subspecies necessarius subsp. nov. and P. necessarius subsp. asymbioticus subsp. nov. Int. J. Syst. Evol. Microbiol. 59: 2002-2009.
  4. ^ Meincke, L., A. Copeland, A. Lapidus, S. Lucas, K. W. Berry, T. Glavina Del Rio, N. Hammon, E. Dalin, H. Tice, S. Pitluck, P. Richardson, D. Bruce, L. Goodwin, C. Han, R. Tapia, J. C. Detter, J. Schmutz, T. Brettin, F. Larimer, M. Land, L. Hauser,, N. C. Kyrpides, N. Ivanova, M. Göker, T. Woyke, Q. L. Wu, M. Pöckl, M. W. Hahn, and H.-P. Klenk (2012). Complete genome sequence of Polynucleobacter necessarius subsp. asymbioticus type strain (QLW-P1DMWA-1T). Standards in Genomic Sciences 6:74-83.
  5. ^ Hahn M. W., Schmidt J., Pitt A., Taipale S. J., Lang E. (2016). Reclassification of four Polynucleobacter necessarius strains as Polynucleobacter asymbioticus comb. nov., Polynucleobacter duraquae sp. nov., Polynucleobacter yangtzensis sp. nov., and Polynucleobacter sinensis sp. nov., and emended description of the species Polynucleobacter necessarius. Int. J. Syst. Evol. Microbiol. 66: 2883–2892.
  6. ^ Hoetzinger M., Hahn M.W., Jezberová J., Schmidt J., Koll U. (2017). Microdiversification of a pelagic Polynucleobacter species is mainly driven by acquisition of genomic islands from a partially interspecific gene pool. Appl. Environ. Microbiol. 83: 3 e02266-16
  7. ^ Hahn, M.W., Scheuerl, T., Jezberová, J., Koll, U., Jezbera, J., Šimek, K., Vannini, C., Petroni, G., and Q. L. Wu (2012) The passive yet successful way of planktonic life: Genomic and experimental analysis of the ecology of a free-living Polynucleobacter population. PLoS ONE 7(3): e32772.
  8. ^ Hoetzinger M., Hahn M.W., Jezberová J., Schmidt J., Koll U. (2017). Microdiversification of a pelagic Polynucleobacter species is mainly driven by acquisition of genomic islands from a partially interspecific gene pool. Appl. Environ. Microbiol. 83: 3 e02266-16