Polysome profiling

Polysome profiling is a technique in molecular biology that is used to study the association of mRNAs with ribosomes. It is important to note that this technique is different from ribosome profiling. Both techniques have been reviewed^[1] and both are used in analysis of the translatome, but the data they generate are at very different levels of specificity. When employed by experts, the technique is remarkably reproducible: the 3 profiles in the first image are from 3 different experiments.^[2]

^ Piccirillo, CA; et al. (2014). "Translational control of immune responses: from transcripts to translatomes". Nature Immunology. 15 (6): 503–511. doi:10.1038/ni.2891. PMID 24840981. S2CID 6269940.
^ Hanebuth, MA; et al. (2016). "Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction". Nature Communications. 7: 13695. Bibcode:2016NatCo...713695H. doi:10.1038/ncomms13695. PMC 5150220. PMID 27917864.

[1] Piccirillo, CA; et al. (2014). "Translational control of immune responses: from transcripts to translatomes". Nature Immunology. 15 (6): 503–511. doi:10.1038/ni.2891. PMID 24840981. S2CID 6269940.

[Hanebuth2016-2] Hanebuth, MA; et al. (2016). "Multivalent contacts of the Hsp70 Ssb contribute to its architecture on ribosomes and nascent chain interaction". Nature Communications. 7: 13695. Bibcode:2016NatCo...713695H. doi:10.1038/ncomms13695. PMC 5150220. PMID 27917864.

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