- 2 September 1347 – Charles IV is crowned King of Bohemia
- 11 September 1951 – Opponents of Czechoslovakia's communist regime escape into West Germany on the Freedom Train
- 23 September 1875 – The first of the trams in Prague (Tatra T3 pictured) gets underway, under the power of horse
- 28 September 935 – Death of St Wenceslaus, marked in the Czech Republic by a national holiday since 2000
- 30 September 1938 – Leaders of Nazi Germany, France, the United Kingdom and Italy sign the Munich Agreement, permitting Germany to annex portions of Czechoslovakia