A prohormone is a committed precursor of a hormone consisting of peptide hormones synthesized together that has a minimal hormonal effect by itself because of its expression-suppressing structure, often created by protein folding and binding additional peptide chains to certain ends, that makes hormone receptor binding sites located on its peptide hormone chain segments inaccessible.[1][2] Prohormones can travel the blood stream as a hormone in an inactivated form, ready to be activated later in the cell by post-translational modification.[1][3]
The body naturally produces prohormones as a way to regulate hormone expression, making them an optimal storage and transportation unit for inactive hormones. Once prohormones are needed to be expressed, prohormone convertase, a protein, cleaves the prohormones and separates them into one or more active hormones.[4] Often in nature, this cleaving process happens immediately, and a prohormone is quickly converted to a set of one or more peptide hormones.[5]
Examples of natural, human prohormones include proinsulin and pro-opiomelanocortin, but the most widespread prohormones in use are synthetic and labeled as anabolic steroid precursors, used as ergogenic or anabolic agents for muscle growth.[6] A commonly consumed example of said precursors are androstenedione and androstenediol, both of which are currently banned substances in the United States.[6][7] However, several illegal steroids, such as 1-testosterone, are still being produced legally under different chemical names, and the majority have not undergone clinical studies.[6][8]