| prostaglandin-F synthase | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Identifiers | |||||||||
| EC no. | 1.1.1.188 | ||||||||
| CAS no. | 55976-95-9 | ||||||||
| Databases | |||||||||
| IntEnz | IntEnz view | ||||||||
| BRENDA | BRENDA entry | ||||||||
| ExPASy | NiceZyme view | ||||||||
| KEGG | KEGG entry | ||||||||
| MetaCyc | metabolic pathway | ||||||||
| PRIAM | profile | ||||||||
| PDB structures | RCSB PDB PDBe PDBsum | ||||||||
| Gene Ontology | AmiGO / QuickGO | ||||||||
| |||||||||
In enzymology, a prostaglandin-F synthase (PGFS; EC 1.1.1.188) is an enzyme that catalyzes the chemical reaction:
- (5Z,13E)-(15S)-9alpha,11alpha,15-trihydroxyprosta-5,13-dienoate + NADP+ (5Z,13E)-(15S)-9alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate + NADPH + H+
Thus, the two products of this enzyme are 9α,11β–PGF2 and NADP+, whereas its three substrates are Prostaglandin D2, NADPH, and H+.
PGFS is a monomeric wild-type protein that was first purified from bovine lung (PDB ID: 2F38).[1] This enzyme belongs to the family of aldo-keto reductase (AKR) based on its high substrate specificity, its high molecular weight (38055.48 Da) and amino acid sequence.[2] In addition, it is categorized as C3 (AKR1C3) because it is an isoform of 3α-hydroxysteroid dehydrogenase.[3]
The function of PGFS is to catalyze the reduction of aldehydes and ketones to their corresponding alcohols. In humans, these reactions take place mostly in the lungs and in the liver.[4] More specifically, PGFS catalyzes the reduction of PGD2 to 9α,11β–PGF2 and PGH2 to PGF2α by using NADPH as cofactor.[2]
- ^ Watanabe K, Yoshida R, Shimizu T, Hayaishi O (June 1985). "Enzymatic formation of prostaglandin F2 alpha from prostaglandin H2 and D2. Purification and properties of prostaglandin F synthetase from bovine lung". The Journal of Biological Chemistry. 260 (11): 7035–41. doi:10.1016/S0021-9258(18)88884-6. PMID 3858278.
- ^ a b Watanabe K (August 2002). "Prostaglandin F synthase". Prostaglandins & Other Lipid Mediators. 68–69: 401–7. doi:10.1016/s0090-6980(02)00044-8. PMID 12432932.
- ^ Komoto J, Yamada T, Watanabe K, Takusagawa F (March 2004). "Crystal structure of human prostaglandin F synthase (AKR1C3)". Biochemistry. 43 (8): 2188–98. doi:10.1021/bi036046x. PMID 14979715.
- ^ Yoshikawa K, Takei S, Hasegawa-Ishii S, Chiba Y, Furukawa A, Kawamura N, et al. (January 2011). "Preferential localization of prostamide/prostaglandin F synthase in myelin sheaths of the central nervous system". Brain Research. 1367: 22–32. doi:10.1016/j.brainres.2010.10.019. PMID 20950588. S2CID 43094318.