Red fluorescent protein

Bioluminescence response to local Ca2+
 after intraperitoneal injection of recombinant tdTA in an anesthetized mouse. An overlay of the mouse reflection image (grayscale) and Ca2+
-induced bioluminescence (red pseudocolor) is shown.
Red fluorescent protein drFP583
Identifiers
OrganismDiscosoma sp.
Symbol?
UniProtQ9U6Y8
Search for
StructuresSwiss-model
DomainsInterPro

Red fluorescent protein (RFP) is a protein which acts as a fluorophore, fluorescing red-orange when excited. The original variant occurs naturally in the coral genus Discosoma, and is named DsRed. Several new variants have been developed using directed mutagenesis[1] which fluoresce orange, red, and far-red.[2]

  1. ^ Bevis, Brooke J.; Glick, Benjamin S. (2002). "Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed)". Nature Biotechnology. 20 (1): 83–87. doi:10.1038/nbt0102-83. ISSN 1546-1696. PMID 11753367. S2CID 20320166.
  2. ^ Miyawaki, Atsushi; Shcherbakova, Daria M; Verkhusha, Vladislav V (October 2012). "Red fluorescent proteins: chromophore formation and cellular applications". Current Opinion in Structural Biology. 22 (5): 679–688. doi:10.1016/j.sbi.2012.09.002. ISSN 0959-440X. PMC 3737244. PMID 23000031.