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[1] The replication factor C, or RFC, is a five-subunit[2] protein complex that is required for DNA replication.
The subunits of this heteropentamer are named Rfc1, Rfc2, Rfc3, Rfc4, and Rfc5 in Saccharomyces cerevisiae. RFC is used in eukaryotic replication as a clamp loader, similar to the γ Complex in Escherichia coli. Its role as a clamp loader involves catalyzing the loading of PCNA onto DNA. It binds to the 3' end of the DNA and uses ATP to open the ring of PCNA so that it can encircle the DNA. ATP hydrolysis causes the release of RFC, with concomitant clamp loading onto DNA. For DNA polymerase, RFC serves as primer identification. RFC plays an important role in the proliferation, invasion, and progression of various malignant tumors. RFC acts as a tumor suppressor gene.
- ^ Tang H, Hilton B, Musich PR, Fang DZ, Zou Y (April 2012). "Replication factor C1, the large subunit of replication factor C, is proteolytically truncated in Hutchinson-Gilford progeria syndrome". Aging Cell. 11 (2): 363–365. doi:10.1111/j.1474-9726.2011.00779.x. PMC 3306506. PMID 22168243.
- ^ Replication+Protein+C at the U.S. National Library of Medicine Medical Subject Headings (MeSH)