A rhabdomyoblast is a cell type which is found in some rhabdomyosarcomas.[1] When found histologically, a rhabdomyoblast aids the diagnosis of embryonal, alveolar, spindle cell/sclerosing, and pleomorphic rhabdomyosarcomas; however, in a tumor, expression of the rhabdomyoblast phenotype is not the only factor in diagnosing a rhabdomyosarcoma.[2][3] Mesenchymal malignancies can exhibit this phenotype as well.[3] Immunohistochemistry techniques allow for the sensitive detection of desmin, vimentin, muscle specific actin, and MyoD1.[4] Similarly the rhabdomyoblast phenotype can be detected morphologically.[3]
Rhabdomyoblasts are early stage mesenchymal cells, having the potential to differentiate into a wide range of skeletal cells.[5] Each stage of differentiation exhibits unique and distinguishable histological characteristics. In its initial form, stellate cells with amphiphilic cytoplasm and ovular central nuclei are observed. Commonly referred to as rhabdoid features, the maturing rhabdomyoblast will likely exhibit low levels of eosinophilic cytoplasm in proximal distances to the nucleus.[6] As maturation and differentiation progress, the cell's cytoplasmic levels of white blood cells increase; additionally, elongated shapes, commonly depicted as “tadpole”, “strap” and "spider cells", are observed. In the concluding phase of differentiation, the white blood cell rich cytoplasm appears bright and exhibits cross-striation.[5] The highly regulated organization of actin and myosin microfilaments in contractile proteins results in this appearance.[7]
With advancements in the medical field, the number of tumors connected to the rhabdomyoblastic phenotype has increased.[3] Recently, the lesion cells of 10 inflammatory tumors were found to possess the rhabdomyoblastic phenotype.[3] Continued research is necessary for precise molecular characterization of the rhabdomyoblastic phenotype and its use in patient case management.[3]
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