Ribosome profiling

Ribosome profiling, or Ribo-Seq (also named ribosome footprinting), is an adaptation of a technique developed by Joan Steitz and Marilyn Kozak almost 50 years ago that Nicholas Ingolia and Jonathan Weissman adapted to work with next generation sequencing that uses specialized messenger RNA (mRNA) sequencing to determine which mRNAs are being actively translated.[1][2] A related technique that can also be used to determine which mRNAs are being actively translated is the Translating Ribosome Affinity Purification (TRAP) methodology, which was developed by Nathaniel Heintz at Rockefeller University (in collaboration with Paul Greengard and Myriam Heiman).[3][2] TRAP does not involve ribosome footprinting but provides cell type-specific information.

  1. ^ Ingolia NT (March 2014). "Ribosome profiling: new views of translation, from single codons to genome scale". Nature Reviews. Genetics. 15 (3): 205–13. doi:10.1038/nrg3645. PMID 24468696. S2CID 13069682.
  2. ^ a b Dougherty, Joseph D. (13 December 2017). "The Expanding Toolkit of Translating Ribosome Affinity Purification". Journal of Neuroscience. 37 (50): 12079–12087. doi:10.1523/JNEUROSCI.1929-17.2017. PMC 5729187. PMID 29237735.
  3. ^ Heiman M, Schaefer A, Gong S, Peterson JD, Day M, Ramsey KE; et al. (2008). "A translational profiling approach for the molecular characterization of CNS cell types". Cell. 135 (4): 738–48. doi:10.1016/j.cell.2008.10.028. PMC 2696821. PMID 19013281.{{cite journal}}: CS1 maint: multiple names: authors list (link)