Route 16 may refer to:
- One of several highways - see List of highways numbered 16
- One of several public transport routes - see List of public transport routes numbered 16
- Mystic Valley Parkway station, also called Route 16 - a proposed light rail station in Medford, Massachusetts
- Route-16 (video game), a 1981 video game
This disambiguation page lists articles associated with the title Route 16.
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