The Samstag Museum is located on the first two levels of the front corner of the building.
The Samstag Museum of Art, also known as the Samstag Museum, was opened in October 2007 as the Anne & Gordon Samstag Museum of Art, in the Hawke Building of the CityWest campus of the University of South Australia (UniSA). The museum is named in honour of Anne & Gordon Samstag, "two distinguished American benefactors to Australian culture, whose remarkable bequest provides opportunities for Australian artists to study overseas."
The gallery had existed in previous incarnations from about 1977, with several names and locations over the next 30 years. In 1991, with the establishment of the University of South Australia, the gallery was renamed the University of South Australia Art Museum, relocating to City West in 1998.