Sequential model

The sequential model (also known as the KNF model) is a theory that describes cooperativity of protein subunits.^[1] It postulates that a protein's conformation changes with each binding of a ligand, thus sequentially changing its affinity for the ligand at neighboring binding sites. It gives one explanation for cooperative binding.

Visual representation of the KNF model in a tetrameric protein.

^ Koshland, D.E., Némethy, G. and Filmer, D. (1966) Comparison of experimental binding data and theoretical models in proteins containing subunits. Biochemistry 5, 365–385. DOI: 10.1021/bi00865a047

[:3-1] Koshland, D.E., Némethy, G. and Filmer, D. (1966) Comparison of experimental binding data and theoretical models in proteins containing subunits. Biochemistry 5, 365–385. DOI: 10.1021/bi00865a047

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