Serratia marcescens nuclease

Serratia marcescens nuclease
Identifiers
EC no.3.1.30.2
CAS no.9025-65-4
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
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NCBIproteins
Serratia marcescens nuclease
Identifiers
OrganismSerratia marcescens
SymbolnucA
UniProtP13717
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StructuresSwiss-model
DomainsInterPro

Serratia marcescens nuclease (EC 3.1.30.2, endonuclease (Serratia marcescens), barley nuclease, plant nuclease I, nucleate endonuclease) is an enzyme.[1][2][3][4] This enzyme catalyses the following chemical reaction

Endonucleolytic cleavage to 5'-phosphomononucleotide and 5'-phosphooligonucleotide end-products

Hydrolyses double- or single-stranded substrate DNA or RNA. It is a representative of the DNA/RNA non-specific endonuclease family.

It is commercially available.

  1. ^ Mikulski AJ, Laskowski M (October 1970). "Mung bean nuclease I. 3. Purification procedure and (3') omega monophosphatase activity". The Journal of Biological Chemistry. 245 (19): 5026–5031. doi:10.1016/S0021-9258(18)62813-3. PMID 4319109.
  2. ^ Stevens A, Hilmoe RJ (1960). "Studies on a nuclease from Azotobacter agilis. I. Isolation and mode of action". Journal of Biological Chemistry. 235 (10): 3016–3022. doi:10.1016/S0021-9258(18)64581-8.
  3. ^ Stevens A, Hilmoe RJ (1960). "Studies on a nuclease from Azotobacter agilis. II. Hydrolysis of ribonucleic and deoxyribonucleic acids". Journal of Biological Chemistry. 235 (10): 3023–3027. doi:10.1016/S0021-9258(18)64582-X.
  4. ^ Wechter WJ, Mikulski AJ, Laskowski M (February 1968). "Gradation of specificity with regard to sugar among nucleases". Biochemical and Biophysical Research Communications. 30 (3): 318–322. doi:10.1016/0006-291x(68)90453-1. PMID 4296679.