Single-strand conformation polymorphism

A single-strand conformation polymorphism gel where DNA was stained with silver staining.

Single-strand conformation polymorphism (SSCP), or single-strand chain polymorphism, is defined as a conformational difference of single-stranded nucleotide sequences of identical length as induced by differences in the sequences under certain experimental conditions. This property allows sequences to be distinguished by means of gel electrophoresis, which separates fragments according to their different conformations.[1]

  1. ^ Masato Orita; Hiroyuki Iwahana; Hiroshi Knazawa; Kenshi Hayashi; Takato Sekiya (1989). "Detection of the polymorphisms of human DNA by gelelectrophoresis as single-strand conformation polymorphisms". Proc. Natl. Acad. Sci. USA. 86 (8): 2766–2770. Bibcode:1989PNAS...86.2766O. doi:10.1073/pnas.86.8.2766. PMC 286999. PMID 2565038.