Solid-phase reversible immobilization

Solid-phase reversible immobilization, or SPRI, is a method of purifying nucleic acids from solution. It uses silica- or carboxyl-coated paramagnetic beads, which reversibly bind to nucleic acids in the presence of polyethylene glycol and a salt. A common application of SPRI technology is purifying samples of DNA amplified by PCR for sequencing reactions:^[1].

^ DeAngelis, M M; Wang, D G; Hawkins, T L (1995-11-25). "Solid-phase reversible immobilization for the isolation of PCR products". Nucleic Acids Research. 23 (22): 4742–4743. doi:10.1093/nar/23.22.4742. ISSN 0305-1048. PMC 307455. PMID 8524672.

[:0-1] DeAngelis, M M; Wang, D G; Hawkins, T L (1995-11-25). "Solid-phase reversible immobilization for the isolation of PCR products". Nucleic Acids Research. 23 (22): 4742–4743. doi:10.1093/nar/23.22.4742. ISSN 0305-1048. PMC 307455. PMID 8524672.

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