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Spontaneous Alternation Behavior (SAB) describes the tendency to alternate in the pursuit of different stimuli in consecutive trials, despite a lack of training or reinforcement.[1][2] The behavior emerged from experiments using animals, mainly rodents, who naturally demonstrated the behavioral pattern when placed in previously unexplored maze shapes (e.g. using a T/Y-maze).[2][3]
Spontaneous alternation testing is a behavioral assessment method derived from SAB. It is used to investigate exploratory behavior[4] and cognitive function (related to spatial learning and memory).[4][5] These assessments are most often done with non-human animals. The test serves great purpose in comparative psychology,[6] wherein non-human animals are studied to investigate differences within and between species with the aims of applying their findings to a greater understanding of human behavior.[7] It is particularly useful in studying the potential neuroanatomical and neurobiological mediators of cognitive function,[8] seeing that there are ethical limitations posed in the physiological study of humans, there is greater opportunity for more invasive procedures to be ethically conducted on non-human animals.