Sucrose lysis test

Sucrose lysis test
Sucrose lysis test
Synonyms	Sucrose hemolysis test
Purpose	screening and diagnosis of paroxysmal nocturnal hemoglobinuria

The sucrose lysis test is a diagnostic laboratory test used for diagnosing paroxysmal nocturnal hemoglobinuria (PNH), as well as for hypoplastic anemias and any hemolytic anemia with an unclear cause.^[1] The test works by using sucrose, which creates a low ionic strength environment that allows complement to bind to red blood cells.^[1] In individuals with PNH, some red blood cells are especially vulnerable to lysis caused by complement.^[1] The test may also produce suspicious results in other hematologic conditions, including megaloblastic anemia and autoimmune hemolytic anemia.^[1] False-negative results can occur when complement activity is absent in the serum.^[1] A simpler alternative called the sugar water test also involves mixing blood with sugar and observing for hemolysis, using the same principle as the sucrose lysis test.^[1]

^ ^a ^b ^c ^d ^e ^f McPherson, Richard A.; Pincus, Matthew R.; Henry, John Bernard (2007). Henry's clinical diagnosis and management by laboratory methods. Philadelphia: Saunders Elsevier. ISBN 978-1-4160-0287-1. OCLC 70327781.

[McPherson_Pincus_Henry_2007_p._519-1] ^ ^a ^b ^c ^d ^e ^f McPherson, Richard A.; Pincus, Matthew R.; Henry, John Bernard (2007). Henry's clinical diagnosis and management by laboratory methods. Philadelphia: Saunders Elsevier. ISBN 978-1-4160-0287-1. OCLC 70327781.

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