Supercritical fluid chromatography

Supercritical fluid chromatography (SFC)[1] is a form of normal phase chromatography that uses a supercritical fluid such as carbon dioxide as the mobile phase.[2][3] It is used for the analysis and purification of low to moderate molecular weight, thermally labile molecules and can also be used for the separation of chiral compounds. Principles are similar to those of high performance liquid chromatography (HPLC); however, SFC typically utilizes carbon dioxide as the mobile phase. Therefore, the entire chromatographic flow path must be pressurized. Because the supercritical phase represents a state whereby bulk liquid and gas properties converge, supercritical fluid chromatography is sometimes called convergence chromatography.[4] The idea of liquid and gas properties convergence was first envisioned by Giddings.[5]

  1. ^ "3.3: Basic Principles of Supercritical Fluid Chromatography and Supercrtical Fluid Extraction". Chemistry LibreTexts. July 13, 2016.
  2. ^ Taylor, Larry T. (2010). "Supercritical Fluid Chromatography". Analytical Chemistry. 82 (12): 4925–4935. doi:10.1021/ac101194x. ISSN 0003-2700. PMID 20465290.
  3. ^ Taylor, Larry T. (2009). "Supercritical fluid chromatography for the 21st century". The Journal of Supercritical Fluids. 47 (3): 566–573. doi:10.1016/j.supflu.2008.09.012. ISSN 0896-8446.
  4. ^ "Beginner's Guide to Convergence Chromatography". Waters.
  5. ^ Giddings, John Calvin (1968). "High Pressure Gas Chromatography of Nonvolatile Species: Compressed gas is used to cause migration of intractable solutes". Science. 162 (3849): 67–73. doi:10.1126/science.162.3849.67. PMID 5675186.