| T-box leader | |
|---|---|
 Predicted secondary structure and sequence conservation of T-box | |
| Identifiers | |
| Symbol | T-box |
| Rfam | RF00230 |
| Other data | |
| RNA type | Cis-reg; leader; |
| Domain(s) | Bacteria |
| GO | GO:0000049 |
| SO | SO:0000140 |
| PDB structures | PDBe |
Usually found in gram-positive bacteria, the T box leader sequence is an RNA element that controls gene expression through the regulation of translation by binding directly to a specific tRNA and sensing its aminoacylation state.[1] This interaction controls expression of downstream aminoacyl-tRNA synthetase genes, amino acid biosynthesis, and uptake-related genes in a negative feedback loop.[1][2] The uncharged tRNA acts as the effector for transcription antitermination of genes in the T-box leader family.[3][4][5] The anticodon of a specific tRNA base pairs to a specifier sequence within the T-box motif, and the NCCA acceptor tail of the tRNA base pairs to a conserved bulge in the T-box antiterminator hairpin.[6]
- ^ a b Grigg JC, Chen Y, Grundy FJ, Henkin TM, Pollack L, Ke A (April 2013). "T box RNA decodes both the information content and geometry of tRNA to affect gene expression". Proceedings of the National Academy of Sciences of the United States of America. 110 (18): 7240–7245. Bibcode:2013PNAS..110.7240G. doi:10.1073/pnas.1222214110. PMC 3645572. PMID 23589841.
- ^ Green NJ, Grundy FJ, Henkin TM (January 2010). "The T box mechanism: tRNA as a regulatory molecule". FEBS Letters. 584 (2): 318–324. doi:10.1016/j.febslet.2009.11.056. PMC 2794906. PMID 19932103.
- ^ Grundy FJ, Rollins SM, Henkin TM (August 1994). "Interaction between the acceptor end of tRNA and the T box stimulates antitermination in the Bacillus subtilis tyrS gene: a new role for the discriminator base". Journal of Bacteriology. 176 (15): 4518–4526. doi:10.1128/jb.176.15.4518-4526.1994. PMC 196270. PMID 8045882.
- ^ Grundy FJ, Collins JA, Rollins SM, Henkin TM (August 2000). "tRNA determinants for transcription antitermination of the Bacillus subtilis tyrS gene". RNA. 6 (8): 1131–1141. doi:10.1017/s1355838200992100. PMC 1369987. PMID 10943892.
- ^ Winkler WC, Grundy FJ, Murphy BA, Henkin TM (August 2001). "The GA motif: an RNA element common to bacterial antitermination systems, rRNA, and eukaryotic RNAs". RNA. 7 (8): 1165–1172. doi:10.1017/s1355838201002370. PMC 1370163. PMID 11497434.
- ^ Gerdeman MS, Henkin TM, Hines JV (February 2003). "Solution structure of the Bacillus subtilis T-box antiterminator RNA: seven nucleotide bulge characterized by stacking and flexibility". Journal of Molecular Biology. 326 (1): 189–201. doi:10.1016/s0022-2836(02)01339-6. PMID 12547201.