TEV protease

nuclear-inclusion-a endopeptidase
TEV protease (white) complexed with peptide substrate (black) with active site triad residues (red). (PDB: 1lvb​)
Identifiers
EC no.3.4.22.44
CAS no.139946-51-3
Databases
IntEnzIntEnz view
BRENDABRENDA entry
ExPASyNiceZyme view
KEGGKEGG entry
MetaCycmetabolic pathway
PRIAMprofile
PDB structuresRCSB PDB PDBe PDBsum
Search
PMCarticles
PubMedarticles
NCBIproteins
Preview warning: Page using Template:Infobox enzyme with unknown parameter "pfam"

TEV protease (EC 3.4.22.44, Tobacco Etch Virus nuclear-inclusion-a endopeptidase) is a highly sequence-specific cysteine protease from Tobacco Etch Virus (TEV).[1] It is a member of the PA clan of chymotrypsin-like proteases.[2] Due to its high sequence specificity, TEV protease is frequently used for the controlled cleavage of fusion proteins in vitro and in vivo.[3] The consensus sequence recognized by TEV protease is Glu-Asn-Leu-Tyr-Phe-Gln-|-Ser, where "|" denotes cleaved peptide bond.[4]

  1. ^ Cite error: The named reference :0 was invoked but never defined (see the help page).
  2. ^ Cite error: The named reference pmid22086950 was invoked but never defined (see the help page).
  3. ^ Kapust RB, Waugh DS (July 2000). "Controlled intracellular processing of fusion proteins by TEV protease". Protein Expr. Purif. 19 (2): 312–8. doi:10.1006/prep.2000.1251. PMID 10873547.
  4. ^ Cite error: The named reference pmid3285343 was invoked but never defined (see the help page).