The Tetbury branch line was a 7.5-mile (12.1 km) single-track branch railway line that connected Tetbury with the main line at Kemble on the line between Swindon and Gloucester.
Opened in 1889, the line's usage declined after 1930 as road competition became stronger. In 1959 diesel railbuses were used for the line's passenger services; this initiative halted the decline, but did not reverse the financial losses on the line, and it closed completely in 1964.