Thiolysis is a reaction with a thiol (R-SH) that cleaves one compound into two.[1] Thiolysis involves the addition of coenzyme A to one of the products. This reaction is similar to hydrolysis, which involves water instead of a thiol.[2] This reaction is seen in β-oxidation of fatty acids.[3] The depolymerisation of condensed tannins with the use of benzyl mercaptan as nucleophile is also called thiolysis.[4]