The Tivoli Theatre was an important venue in Melbourne's East End Theatre District, located at 249 Bourke Street near Swanston Street. The first theatre on the site opened in 1866, rebuilt in 1872 as the Prince of Wales Opera House, rebuilt again in 1901 as the New Opera House, and renamed the Tivoli in 1914 when it joined the Tivoli circuit. The theatre closed in 1966.