TopHat (bioinformatics)

TopHat is an open-source bioinformatics tool for the throughput alignment of shotgun cDNA sequencing reads generated by transcriptomics technologies (e.g. RNA-Seq) using Bowtie first and then mapping to a reference genome to discover RNA splice sites de novo.[1] TopHat aligns RNA-Seq reads to mammalian-sized genomes.[2]

  1. ^ Trapnell C, Pachter L, Salzberg SL (May 2009). "TopHat: discovering splice junctions with RNA-Seq". Bioinformatics. 25 (9): 1105–11. doi:10.1093/bioinformatics/btp120. PMC 2672628. PMID 19289445.
  2. ^ "TopHat". ccb.jhu.edu. Retrieved 2018-04-17.