The Treaty of Holston (or Treaty of the Holston) was a treaty between the United States government and the Cherokee signed on July 2, 1791, and proclaimed on February 7, 1792. It was negotiated and signed by William Blount, governor of the Southwest Territory and superintendent of Indian affairs for the southern district of the United States, and various representatives of the Cherokee peoples, most notably John Watts. The treaty established terms of relations between the United States and the Cherokee established that the Cherokee tribes were to fall under the protection of the United States, with the United States managing all future foreign affairs for all the loosely affiliated Cherokee tribes.
A monument to the treaty, erected in 1997, is located on the banks of the Tennessee River in downtown Knoxville, Tennessee, where the treaty was negotiated.