Tsukui District (津久井郡, Tsukui-gun) was a district located in the far northwestern corner of Kanagawa Prefecture, Japan.
From 2007 it merged into the city of Sagamihara. Prior to its merger it consisted of four towns (Shiroyama, Tsukui, Sagamiko, Fujino), with a total area of 238.44 square kilometers and a population of 72,871. The district was located in the Tanzawa Mountains.