Two-dimensional gel electrophoresis

2D-Gels (Coomassie stained)
Robots are used for the isolation of protein spots from 2D gels in modern laboratories.

Two-dimensional gel electrophoresis, abbreviated as 2-DE or 2-D electrophoresis, is a form of gel electrophoresis commonly used to analyze proteins. Mixtures of proteins are separated by two properties in two dimensions on 2D gels. 2-DE was first independently introduced by O'Farrell[1] and Klose[2] in 1975.

  1. ^ O'Farrell, PH (1975). "High resolution two-dimensional electrophoresis of proteins". J. Biol. Chem. 250 (10): 4007–21. doi:10.1016/S0021-9258(19)41496-8. PMC 2874754. PMID 236308.
  2. ^ Klose, J (1975). "Protein mapping by combined isoelectric focusing and electrophoresis of mouse tissues. A novel approach to testing for induced point mutations in mammals". Humangenetik. 26 (3): 231–43. doi:10.1007/bf00281458. PMID 1093965. S2CID 30981877.