U.S. Highway 301 | ||||
|---|---|---|---|---|
 US 301 highlighted in red | ||||
| Route information | ||||
| Auxiliary route of US 1 | ||||
| Maintained by FDOT | ||||
| Length | 269.316 mi[1] (433.422 km) | |||
| Existed | 1949–present | |||
| Major junctions | ||||
| South end |  US 41 in Sarasota | |||
| North end |      US 1 / US 23 / US 301 / SR 4 / SR 15 south of Folkston, Georgia | |||
| Location | ||||
| Country | United States | |||
| State | Florida | |||
| Counties | Sarasota, Manatee, Hillsborough, Pasco, Hernando, Sumter, Marion, Alachua, Bradford, Clay, Duval, Nassau | |||
| Highway system | ||||
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U.S. Route 301 (US 301) in Florida runs from the Sarasota-Bradenton-Venice, Florida Metropolitan Statistical Area northeast to the Greater Jacksonville Metropolitan Area. The road is a spur of U.S. Route 1, which it intersects in Callahan.
US 301 carries the hidden state road designations of State Road 683 from Sarasota to US 41 in Bradenton, SR 55 from Bradenton to Palmetto, SR 43 from US 41 in Palmetto to US 92 east of Tampa, SR 41 from US 92 to Zephyrhills, SR 39 from Zephyrhills to US 98 south of Dade City, State Road 35 from US 98 to Belleview (except SR 533 around downtown Dade City), SR 500 from Belleview to Ocala, SR 200 from Ocala to Callahan and SR 15 from Callahan to the Georgia border, south of Folkston, Georgia.
Concurrencies include U.S. 41 between Bradenton and Palmetto, U.S. 98 between Clinton Heights (near Dade City) and Trilacoochee (formerly known as Moss Town), US 27 between Belleview and Ocala, US 441 between Belleview and Sparr, US 90 in Baldwin, and U.S. 1 and US 23 north of Callahan.