User:Bfigura/Sandbox-Kunkel

Sandbox Version of an article on the Kunkel Method, an expansion from Site-directed_mutagenesis. I'm using some content from there to start the article. It'll be expanded upon before creation.

The Kunkel Method is a classic [1] site-directed mutagenesis technique used in molecular biology to cause a mutation at a defined site in a circular DNA molecule, known as a plasmid. While it is not currently the most efficient method available, it is notable for being the first method that eliminated the need for phenotypic expression[2]. The method was developed by TA Kunkel in 1985[2], and takes advantage of the fact that uracil (a base normally found in RNA, not DNA) will not translate properly if recombinantly introduced into a bacteria (such as E. coli).[3]

  1. ^ Brown, K. D.; McKay, Ian A. (1998). Growth factors and receptors: a practical approach. Oxford [Oxfordshire]: Oxford University Press. p. 22. ISBN 0-19-963646-X.{{cite book}}: CS1 maint: multiple names: authors list (link)
  2. ^ a b Kunkel TA (1985). "Rapid and efficient site-specific mutagenesis without phenotypic selection". Proc. Natl. Acad. Sci. U.S.A. 82 (2): 488–92. PMID 3881765. {{cite journal}}: |access-date= requires |url= (help)
  3. ^ Michael Smith. Synthetic DNA and Biology. Nobel Lecture, December 8, 1993. Smith Lecture on NobelPrize.org