Walkerville Brewery was a brewer of beer in Adelaide, South Australia, originally founded in the 1840s. The company became a co-operative, and grew by admitting hotel owners as shareholders, and absorbed smaller breweries. After several amalgamations it moved its operations to Southwark (now part of Thebarton) and by 1920 it was South Australia's largest brewing company.
It was bought out by the South Australian Brewing Company in 1939 and its facilities became known as the company's Southwark brewery. The brewery produced Nathan beer (Nathan Bitter), named after a new type of beer making equipment introduced in 1927 (later renamed Southwark Bitter by the SA Brewing Co.).