William Louis of Anhalt-Harzgerode (Harzgerode, 18 August 1643 – Harzgerode, 14 October 1709), was a German prince of the House of Ascania and the last ruler of the principality of Anhalt-Harzgerode.
He was the eldest child and only son of Frederick, Prince of Anhalt-Harzgerode, by his first wife Johanna Elisabeth, daughter of John Louis, Prince of Nassau-Hadamar.